SNP Typing Using Multiplex Real-Time PCR Assay for Species Identification of Forensically Important Blowflies and Fleshflies Collected in South Korea (Diptera: Calliphoridae and Sarcophagidae)

<div>Melt curves of amplicons using primer combinations (Calliphoridae). (a) CC1: (1) P. regina (Tm = 74.29°C). (2) L. illustris (Tm = 77.25°C). (3) L. caesar (Tm = 77.25°C). (4) C. megacephala (Tm = 79.15°C). (b) CC2: (1) L. ampullacea (Tm = 73.54°C). (2) C. lata (Tm = 75.91°C). (3) L. caesar (Tm = 78.88°C). (c) CC3: (1) C. vicina (Tm = 72.95°C). (2) L. ampullacea (Tm = 75.76°C). (3) L. ampullacea (nonamplified). (4) A. grahami (Tm = 76.06°C). (5) C. vomitoria (Tm = 74.87°C). (d) CC4: (1) C. pinguis (Tm = 74.87°C). (2) L. sericata (Tm = 76.80°C). (3) T. calliphoroides (Tm = 79.77°C). (4) C. vicina (Tm = 76.32°C). (5) C. vicina (nonamplified).</div>

BioMed Research International

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Figure 1: SNP Typing Using Multiplex Real-Time PCR Assay for Species Identification of Forensically Important Blowflies and Fleshflies Collected in South Korea (Diptera: Calliphoridae and Sarcophagidae) 

Figure 1 | SNP Typing Using Multiplex Real-Time PCR Assay for Species Identification of Forensically Important Blowflies and Fleshflies Collected in South Korea (Diptera: Calliphoridae and Sarcophagidae) 