Research Article

SNP Typing Using Multiplex Real-Time PCR Assay for Species Identification of Forensically Important Blowflies and Fleshflies Collected in South Korea (Diptera: Calliphoridae and Sarcophagidae)

Figure 2

Melt curves of amplicons using primer combinations (Sarcophagidae). (a) Melt curves of amplicons generated by primer combination CS1. Number of figure and species amplified, from left to right, are as follows: a-1, S. peregrina (Tm = 75.61°C); a-2, L. melanura (Tm = 80.21°C). (b) Melt curves of amplicons generated by primer combination CS2. Number of figure and species amplified, from left to right, are as follows: b-1, S. dux (Tm = 74.87°C); b-2, S. similis (Tm = 78.13°C). (c) Melt curves of amplicons generated by primer combination CS3. Number of figure and species amplified, from left to right, are as follows: c-1, S. haemorrhoidalis (Tm = 73.98°C); c-2, S. crassipalpis (Tm = 75.77°C); c-3, S. albiceps (Tm = 79.47°C).
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