SNP Typing Using Multiplex Real-Time PCR Assay for Species Identification of Forensically Important Blowflies and Fleshflies Collected in South Korea (Diptera: Calliphoridae and Sarcophagidae)

Table 2

Information on species-specific primers (Calliphoridae).

Primer Set	Forward/Reverse	Sequence ()	Amplicon Size; (bp)	Target Species
gr	F	TGA CTT CTA CCC CCT GCA C	76	A. graham; (L. ampullacea)
	R	CAG TTC ATC CTG TTC CAG CTC
la	F	GTA GTT GCT CAT TTT CAT TAT; GTG C	77	C. lata
	R	GGG TAT CAG TGT ACA AAT CCT; GCT
vi	F	CTG TCC CAA CAG GAA TTA AGA; TTT TT	56	C. vicina
	R	TTG GGT ACC ATA AAG AGT TGC T
vo	F	ATA CGA TCA ACA GGT ATT ACC; TTC	163	C. vomitoria
	R	CTC CTG CTG GGT CAA AGA AT
mg	F	CTT TGA TCC AGC AGG AGG AG	265	Ch. megacephala
	R	TGA AGT GAA ATA AGC TCG TGT; GTC T
pi	F	CGT AGA TAC TCG AGC TTA TTT; CAC C	65	Ch. pinguis
	R	TCT TAA TTC CAG TTG GTA CAG; CA
am	F	TTC TAC AAT TGG ATC AAC AAT; TTC TC	76	L. ampullacea
	R	AAC AAG ACT TTC TCA CAC AAT; AAA GAA
ca	F	GGA AAG AAG GAA ACA TTT GGG	207	L. caesar
	R	TTG AGT TCC GTA AAG AGT TGC T
il/ca	F	GGA TTC GTT CAT TGA TAT CCT C	145	L. caesar; L. illustris
	R	ATC GTC GTG GTA TTC CTG CT
se	F	GAA ACA TTC GGT TCA TTA GGG; ATG	113	L. sericata
	R	GCT CGT GTA TCA ACG TCT ATT CC
re	F	TTG GAG GAT TTG GAA ATT GA	61	P. regina
	R	GGG AAA GCT ATA TCA GGT GCC
cl	F	ATA TGG CTT TCC CTC GGA TAA; AC	151	T. calliphoroides; (C. vicina)
	R	GCT CCT CCA TGT GCG ATA TT

: L. ampullacea may or may not be amplified with primer set “gr” due to variation.
: L. illustris and L. caesar are both amplified with primer set “il/ca.”
: C. vicina may or may not be amplified with primer set “cl” due to variation.