SNP Typing Using Multiplex Real-Time PCR Assay for Species Identification of Forensically Important Blowflies and Fleshflies Collected in South Korea (Diptera: Calliphoridae and Sarcophagidae)

Table 3

Information on species-specific primers (Sarcophagidae).

Primer Set	Forward/Reverse	Sequence ()	Amplicon Size; (bp)	Target Species
al	F	TTT TTG GAG CTT GAG CAG GT	231	S. albiceps
	R	TTA TTC GAG GGA ATG CCA TG
cr	F	ACC GGT ACT TGC AGG AGC TA	74	S. crassipalpis
	R	CTG CTG GGT CGA AAA AAG AG
du	F	CTT TTA CCA CCA GCA TTA ACA; TTA TTA	68	S. dux
	R	CAT CCT GTT CCA GCT CCA TT
hm	F	CAA AAT ACA CCA CCA GCT GAA	65	S. haemorrhoidalis; = S. africa
	R	tta gaT TAG AAG TTA GTT AGT; AGC GGT
me	F	GAG CCC CAG ATA TAG CTT TCC	208	S. melanura
	R	GAA GAG ATT CCA GCT AGA TGA; AG
pg	F	TAT GGC CGG ATT TGT TCA TT	135	S. peregrina
	R	GCT AAA CCT AAG AAG TGT TGG; GGA
si	F	TAC TTC ATT CTT TGA TCC TGC T	159	S. similis
	R	CCA AAT GTT TCC TTT TTA CCT; GA

: Primer set “hm” was designed based on the sequences of COI and tRNA. Lowercase letters indicate sequences from tRNA.