SNP Typing Using Multiplex Real-Time PCR Assay for Species Identification of Forensically Important Blowflies and Fleshflies Collected in South Korea (Diptera: Calliphoridae and Sarcophagidae)

Table 5

Combinations of species-specific primers (Sarcophagidae).

Combination Name	Primer Set	Sequence ()	Optimized Concentration; (μM)	Target Species
CS1	me	GAG CCC CAG ATA TAG CTT; TCC; GAA GAG ATT CCA GCT AGA; TGA AG	0.10	S. melanura
	pg	TAT GGC CGG ATT TGT TCA TT; GCT AAA CCT AAG AAG TGT; TGG GGA	0.10	S. peregrina
CS2	du	CTT TTA CCA CCA GCA TTA; ACA TTA TTA; CAT CCT GTT CCA GCT CCA TT	0.15	S. dux
	si	TAC TTC ATT CTT TGA TCC; TGC T; CCA AAT GTT TCC TTT TTA; CCT GA	0.10	S. similis
CS3	al	TTT TTG GAG CTT GAG CAG; GT; TTA TTC GAG GGA ATG CCA; TG	1.20	S. albiceps
	cr	ACC GGT ACT TGC AGG AGC; TA; CTG CTG GGT CGA AAA AAG; AG	0.15	S. crassipalpis
	hm	CAA AAT ACA CCA CCA GCT; GAA; TTA GAT TAG AAG TTA GTT; AGT AGC GGT	0.10	S. haemorrhoidalis