SNP Typing Using Multiplex Real-Time PCR Assay for Species Identification of Forensically Important Blowflies and Fleshflies Collected in South Korea (Diptera: Calliphoridae and Sarcophagidae)

Table 6

Obtained melting temperatures (T_m) of amplicons using primer combinations (Calliphoridae).

Combination Name	Amplified Species	Tm of Amplicon; (°C)
CC1	P. regina	74.43±0.05
	L. illustris	76.95±0.37
	L. caesar	77.02±0.37
	Ch. megacephala	79.40±0.38
CC2	L. ampullaceal	73.74±0.80
	C. lata	75.84±0.52
	L. caesar	78.58±0.30
CC3	C. vicina	72.85±0.79
	L. ampullaceal	75.90±0.14; or non-amplified
	A. graham	75.92±0.29
	C. vomitoria	77.69±0.30
CC4	Ch. pinguis	74.86±0.42
	L. sericata	76.63±0.45
	T. calliphoroides	79.84±0.53
	C. vicina	79.40±0.08; or non-amplified