FOXO3 Is Expressed in Ovarian Tissues and Acts as an Apoptosis Initiator in Granulosa Cells of Chickens

Figure 4

Treatment of chicken granulosa cells with the exogenous recombinant FOXO3 protein. (a) Western blot analysis of FOXO3 protein levels in extracted nuclear lysates; the relative expression level was normalized to that of H3. (b) The expression of apoptosis-related genes in chicken granulosa cells after treatment with different concentrations of the exogenous recombinant FOXO3 protein. (c) Western blot analysis showing the expression levels of PARP-1 and cleaved caspase 3 after recombinant-FOXO3 treatment. β-Actin served as a reference. Data are presented as means ± SEM (n = 3 independent cell cultures). P<0.05; P<0.01.

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