Label-Free LC-MS/MS Proteomics Analyses Reveal Proteomic Changes Accompanying MSTN KO in C2C12 Cells

Figure 1

Mass spectrometry-based quantitative proteomic analysis of skeletal muscle C2C12 cell proteins. Volcano plot of a total of 3003 quantified proteins illustrating increased and decreased expression following MSTN knockout. The horizontal coordinate is the difference multiple (logarithmic transformation at the base of 2), and the vertical coordinate is the significant difference p value (logarithmic transformation at the base of 10). Red dots represent the expression profiles of 77 significantly different proteins showing a fold change ≥ ± 2 in their relative abundance in MSTN KO cells relative to control cells.