IGHG1 Regulates Prostate Cancer Growth via the MEK/ERK/c-Myc Pathway
Figure 3
IGHG1 regulates PCa cell growth via MEK/ERK/c-Myc pathway. PCa tissue samples were subjected to immunohistochemistry; the representative images of IGHG1 and c-Myc expression were shown in (a) and the expression levels between the two proteins were shown in (b). DU145 cells (c, g) and PC3 cells (e, j) were transfected with IGHG1 siRNA fragment or si-Ctrl or with IGHG1 antibody and were subjected to western blot with c-Myc, Cyclin D1, p21, phosphor-MEK, total MEK, phosphor-ERK, and total ERK antibodies. GAPDH was used as loading control. The statistical data of expression of c-Myc, Cyclin D1, and p21 proteins (comparing to GAPDH) were shown in (d) and (f). The statistical data of p-MEK (comparing to total MEK, (h) and (k)) and p-ERK (comparing to total ERK, (i) and (l)) were shown as the mean ± SD. denotes p < 0.05 versus si-Ctrl group; # denotes p < 0.05 versus normal control group.