Cox-2 Negatively Affects the Protective Role of Propofol against Hypoxia/Reoxygenation Induced Cardiomyocytes Apoptosis through Suppressing Akt Signaling

Figure 3

Over-expression of Cox-2 suppressed the protective effect of propofol. (a) Relative mRNA expression of Cox-2 in Cox-2 over-expressed H9c2 cardiomyocytes. (b) Western blot analysis of Cox-2 expression in Cox-2 over-expressed H9c2 cardiomyocytes. (c) Cell viability and (d) LDH release in H9c2 cardiomyocytes under indicated conditions. NC means negative control; OE means over-expression; HR means H/R treatment group; P means treatment of propofol; Cox-2 means Cox-2 over-expression; SC79 means treatment of SC79 (10 μM). Data were presented as the mean ± SD (n ≥ 3). P<0.05, P<0.01, P<0.001.

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