The Mycobacterium tuberculosis CRISPR-Associated Cas1 Involves Persistence and Tolerance to Anti-Tubercular Drugs

Figure 2

Expression of Cas1 changes the morphology and growth kinetic of M. smegmatis. (a) PCR amplification of Cas1 encoding gene (1016 bp) using mc² 155_Vec (lane 1) and mc² 155_Cas1 (lane 2). (b) Expression of Cas1 protein was measured by Western blotting of total lysate. (c) Morphological differences between mc² 155_Vec and mc² 155_Cas1 single clone grown on 7H10 agar plates. (d) Growth Kinetics of mc² 155_Vec and mc² 155_Cas1. Error bars indicate standard deviation of triplicate experiments. P<0.05; P<0.01.

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