Magnesium Enhances Osteogenesis of BMSCs by Tuning Osteoimmunomodulation

Table 1

Primer pairs used in the qRT-PCR.

Gene	Primer sequences
CD206	Forward: 5′-AGACGAAATCCCTGCTACTG-3′
	Reverse: 5′-CACCCATTCGAAGGCATTC-3′
CCR7	Forward: 5′-ATGACGTCACCTACAGCCTG-3′
	Reverse: 5′-CAGCCCAAGTCCTTGAAGAG-3′
IL-1ra	Forward: 5′-CTCCAGCTGGAGGAAGTTAAC-3′
	Reverse: 5′-CTGACTCAAAGCTGGTGGTG-3′
IL-10	Forward: 5′-GAGAAGCATGGCCCAGAAATC-3′
	Reverse: 5′-GAGAAATCGATGACAGCGCC-3′
IL-1β	Forward: 5′-TGGAGAGTGTGGATCCCAAG-3′
	Reverse: 5′-GGTGCTGATGTACCAGTTGG-3′
IL-6	Forward: 5′-ATAGTCCTTCCTACCCCAATTTCC-3′
	Reverse: 5′-GATGAATTGGATGGTCTTGGTCC-3′
IL-18	Forward: 5′-TGGCCGACTTCACTGTACAAC-3′
	Reverse: 5′-TGGGGTTCACTGGCACTTTG-3′
TNF-α	Forward: 5′-CTGAACTTCGGGGTGATCGG-3′
	Reverse: 5′-GGCTTGTCACTCGAATTTTGAGA-3′
Myd88	Forward: 5′-AGGTAAGCAGCAGAACCAGG -3′
	Reverse: 5′-TGTCCTAGGGGGTCATCAAGG-3′
Ticam1	Forward: 5′-AGATGGTTCAGCTGGGTGTC-3′
	Reverse: 5′-TGGAGTCTCAAGAAGGGGTTC-3′
Ticam2	Forward: 5′-CTTGGCGCTGCAAACCATC-3′
	Reverse: 5′-GCCTCTCAAATACAGACTCCCG-3′
TGF-β1	Forward: 5′-GTGGAAATCAACGGGATCAGC-3′
	Reverse: 5′-CAGCAGTTCTTCTCTGTGGAGC-3′
TGF-β3	Forward: 5′-CAACACCCTGAACCCAGAG-3′
	Reverse: 5′-CTT CACCACCATGTTGGACAG-3′
BMP-2	Forward: 5′-GCTCCACAAACGAGAAAAGC-3′
	Reverse: 5′-AGCAAGGGGAAAAGGACACT-3′
BMP-6	Forward: 5′-TGGCAGGACTGGATCATTGC-3′
	Reverse: 5′-ACCAAGGTCTGTACAATGGCG-3′
VEGF	Forward: 5′-GTCCCATGAAGTGATCAAGTTC-3′
	Reverse: 5′-TCTGCATGGTGATGTTGCTCTCTG-3′
GAPDH (mouse)	Forward: 5′-TGACCACAGTCCATGCCATC-3′
	Reverse: 5′-GACGGACACATTGGGGGTAG-3′
Runx-2	Forward: 5′-TCTTTTGGGATCCGAGCACC-3′
	Reverse: 5′-ATCTCCACCATGGTGCGGTT-3′
ALP	Forward: 5′-CCA TTT CAG CCT CAG GAT CG-3′
	Reverse: 5′-TGG CCA CGT TGG TGT TGA GT-3′
OPN	Forward: 5′-CCAAGCGTGGAAACACACAGCC-3′
	Reverse: 5′-GGCTTTGGAACTCGCCTGACTG-3′
OCN	Forward: 5′-GCCCTGACTGCATTCTGCCTCT-3′
	Reverse: 5′-TCACCACCTTACTGCCCTCCTG-3′
SMAD4	Forward: 5′- TACCACCATAACAGCACTAC-3′
	Reverse: 5′-GAACACCAATATTCAGGAGC-3′
SMAD5	Forward: 5′-GTACTATGAACTGAACAACGG-3′
	Reverse: 5′-TATAGATGGACACCTTTCCC-3′
SMAD1	Forward: 5′-GAGATCAATAGAGGAGATGTTC -3′
	Reverse: 5′-TCGGTTCTTATTGTTGGAAG-3′
BMPR1A	Forward: 5′-GACACGTGCGAATTGGACAATG-3′
	Reverse: 5′-CGTCTGATTTCATACCAGTAC-3′
GAPDH (rat)	Forward: 5′-TCAGCAATGCCTCCTGCAC-3′
	Reverse: 5′-TCTGGGTGGCAGTGATGGC-3′