Production Optimization of an Active -Galactosidase of Bifidobacterium animalis in Heterologous Expression Systems

Figure 2

Biochemical characterization of recombinant bG42-106 from E. coli. (a) Effects of pH on the enzyme activity. (b) Effects of temperature on the enzyme activity. (c) pH stability of bG42-106. (d) Thermal stability of bG42-106. Closed squares, 100 mM Na₂HPO₄-citric acid (pH 3.0–7.0); closed triangles, 100 mM Tris-HCl (pH 7.0–9.0); closed circles, 100 M Na₂CO₃-NaHCO₃ (pH 9.0–10.0). The error bars represent the mean ± standard deviation of three replicates.

(a)

(b)

(c)

(d)