Research Article

Production Optimization of an Active -Galactosidase of Bifidobacterium animalis in Heterologous Expression Systems

Table 1

Primers used in this study.

PrimersSequences (5′ 3′)

bG42FGACTACWACCCNGANCANTG
bG42RGTATTCRTTSTYNAYSTGCC
Dsp1ACATCGTATCCCTGGCCATCTTCAG
Dsp2ACCTCGCATCCGCCACCGCTTC
Dsp3TCCGACATTCCGCACATACGCCC
Usp1AGGGCGTATGTGCGGAATGTCGG
Usp2AAGCGGTGGCGGATGCGAGGTC
Usp3CTGAAGATGGCCAGGGATACGATG
bG42-106fGCGCCCTAGGGATATCATGTCAGCATCCACACAACATCGTG
bG42-106rTATAGCGGCCGCGCGCCTGAACGCCAGAACGCCGTTTG
P1CGTTTGGATCCTTCGAATAATTAGTTG
P2CGGTCTCAGAAAAAGCATAAACAGTTCTAC
P3AAAAGAGAGGCTGAAGCTATGAAAGCAAATATCAAATGGC
P4TATGCTTTTTCTGAGACCGCAAAGTTGGTAGATGTGA
CherryFCGAAGGATCCAAACGATGGCAGAACAAAGCGAC
CherryRTCAGCCTCTCTTTTCTCAAGGGTTTCCGAAG
ScPDIfATGAATTCATGAAGTTTTCTGCTGGTGCCGTC
ScPDIrATGCGGCCGCTTACAATTCATCGTGAATGG

Y represents T or C, W for A or T, S for C or G, R for A or G, N for A, T, C, or G; restriction sites are italic; overlapping sequences are underlined.