Production Optimization of an Active -Galactosidase of Bifidobacterium animalis in Heterologous Expression Systems

Table 2

Effect of metal ions and chemical reagents on the β-galactosidase activity of purified recombinant bG42-106.

	Relative activity (%)
Metal ions and reagents	1 mM	10 mM
Control	100.0	100.0
Na^+	97.9 ± 1.0	95.6 ± 2.0
K^+	98.6 ± 2.0	94.4 ± 1.0
Ca^2+	97.6 ± 3.0	97.5 ± 1.0
Cu^2+	90.6 ± 1.0	44.3 ± 4.0
Mn^2+	100.9 ± 3.0	93.2 ± 2.0
Co^2+	99.4 ± 2.0	96.0 ± 1.0
Cd^2+	96.7 ± 2.0	59. 3 ± 2.0
Fe^2+	96.9 ± 1.3	65.3 ± 3.0
Ni^2+	102.3 ± 1.0	96.2 ± 0
Mg^2+	102.3 ± 2.0	87.2 ± 1.0
Zn^2+	104.2 ± 2.0	95.4 ± 2.0
Ag^+	0	0
Pb^2+	0	0
Triton	103.2 ± 4.0	94.1 ± 2.0
EDTA	100.3 ± 3.0	91.6 ± 2.0
SDS	55.5 ± 2.0	39.3 ± 4.0
CTAB	90.8 ± 1.0	91.8 ± 2.0

Values represent the means of triplicates relative to the untreated control samples.