| | Author, Year | Cancer type | Total number | Calpain-4 protein expression | Judgment standards for high Capn4 expression | Outcome measures | Follow-ups | Analysis | HR (95%CI) for OS | HR (95%CI) for DFS/PFS/RFS | NOS | | H/P | L/N |
| | Cheng F, 2018 | CRC | 132 | 87 | 45 | Each specimen was scored for the extent of stained cells (0-1% = 0, 1~24% =1, 25~49% =2, 50~74% =3, 75~100% =4). A value ≥2 as a high score. | OS, DFS | ≥5 years | UVA | 1.91 (1.21-2.78) | 1.85 (1.12-2.39) | 6 |
| | Wu XX, 2018 | ESCC | 155 | 105 | 50 | The final score was obtained by multiplying the ratio of positive cells score and the intensity score, and a final score of 8-12 was classified as high expression. | OS | ≥5 years | MVA | 1.68 (1.05-3.97) | NR | 7 |
| | Yang MF, 2018 | OC | 113 | 60 | 53 | The final scores of Capn4 expression,
ranging from 0 to 9, were calculated by multiplying the percentage score by the intensity score. A final score of ≥4 was classified as high expression. | OS, PFS | ≥5 years | MVA | 2.16 (1.09-3.14) | 2.04 (1.28-3.27) | 7 |
| | Yang X, 2017 | OC | 91 | 68 | 23 | The staining index was evaluated by multiplying the score of staining intensity and the percentage of positive tumor cells, samples with an SI ≥8 were defined as showing high expression. | OS | ≥5 years | UVA | 1.62 (1.10-2.37) | NR | 6 |
| | Peng P, 2016 | GC | 174 | 102 | 72 | Composite expression scores = 4 (intensity score−1) + frequency score.
CES of calpain-4 greater than or equal to 6 were considered to be high expression. | OS | ≥5 years | MVA | 1.98 (1.16-3.38) | NR | 8 |
| | Zhang C, 2013 | ICC | 140 | 80 | 60 | Capn4 high: the mean area of positive
staining>50% of the tumor section. | OS, RFS | ≥5 years | MVA | 1.66 (1.12-2.46) | 1.49 (1.01-2.21) | 7 |
| | Zheng PC, 2014 | NPC | 153 | 72 | 61 | The scores of 0, 1-2, 3-4, and 5-6 were considered to be negative, low, medium, and strong, respectively. The scores of 5–6 were considered to be high. | OS, PFS | ≥5 years | UVA | 1.56 (1.04-3.01) | 1.63 (1.11-3.08) | 7 |
| | Gu J, 2015 | NSCLC | 208 | 111 | 97 | The intensity of Capn4 was classified into four grades (0 for negative; 1 for weak; 2 for moderate; and 3 for strong). Scores of 2 or 3 were considered Capn4 high. | OS | ≥5 years | MVA | 1.53 (1.04-2.25) | NR | 8 |
| | Bai DS, 2009 | HCC | 192 | 70 | 122 | The final score of each sample was assessed by summarizing the result of intensity and extent of staining. The case was considered positive if the final score was
4 to 5 (+) or 6 to 7 (++). | OS, RFS | ≥5 years | MVA | 4.07 (2.52-6.55) | 46.95 (13.96-157.93) | 8 |
| | Cai JJ, 2014 | GBM | 94 | 52 | 42 | A positive reaction for Capn4 was classified into four grades (0 for negative; 1 for weak; 2 for moderate; and 3 for strong). The moderate or strong intensity was classified as high Capn4 expression. | OS, PFS | ≥5 years | MVA | 1.54 (1.00-2.06) | 1.83 (1.17-2.85) | 6 |
| | Dai Z, 2014 | HCC | 323 | 161 | 162 | NR | OS | ≥5 years | MVA | 1.87 (1.35-2.60) | NR | 8 |
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