All-Trans Retinoic Acid Enhances Bacterial Flagellin-Stimulated Proinflammatory Responses in Human Monocyte THP-1 Cells by Upregulating CD14

Figure 7

Anti-CD14 antibody treatment suppresses flagellin-induced TNF-α and IL-1β secretion of ATRA-treated THP-1 cells. (a) THP-1 cells were treated with 1 μg/ml anti-CD14 antibody (My4) for 30 min prior to treatment with 1 μM ATRA or DMSO for 24 h and then stimulated with 100 ng/ml of flagellin from S. typhimurium for 24 h; the cell culture supernatant was collected and subjected to ELISA for detecting TNF-α production. (b) THP-1 cells were treated with 1 μg/ml anti-CD14 antibody (My4) for 30 min prior to treatment with 1 μM ATRA or DMSO for 24 h and then stimulated with 100 ng/ml flagellin for 24 h; the cell culture supernatant was collected and subjected to ELISA detecting for IL-1β production. Bar graphs indicate secreted cytokine amount ± SD. Statistical significance was assessed by one-way ANOVA using SPSS. vs. DMSO control; vs. ATRA group.

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