LncRNA HOXA11-AS Promotes Proliferation and Cisplatin Resistance of Oral Squamous Cell Carcinoma by Suppression of miR-214-3p Expression
Figure 2
HOMA11-AS facilitated proliferation and CDDP resistance in OSCC cells. (a) TSCCA-CDDP and CAL-27-CDDP cells were transfected with si-HOMA11-AS or its negative control, and the transfection efficiency was confirmed by real-time PCR (p=0.021; p=0.024). (b) TSCCA and CAL-27 cells were transfected with pcDNA-HOMA11-AS or its negative control, and the transfection efficiency was confirmed by real-time PCR (p=0.0008; p=0.0009). ((c) and (d)) Cell proliferation in TSCCA-CDDP and CAL-27-CDDP cells transfected by si-HOMA11-AS or its negative control (p=0.017; p=0.022). (e) IC50 value of CDDP in TSCCA-CDDP and CAL-27-CDDP cells transfected by si-HOMA11-AS or its negative control (p=0.025; p=0.031). ((f) and (g)) Cell proliferation in TSCCA and CAL-27 cells transfected by pcDNA-HOMA11-AS or its negative control (p=0.019; p=0.017). (h) IC50 value of CDDP in TSCCA and CAL-27 cells transfected by pcDNA-HOMA11-AS or its negative control (p=0.032; p=0.038). (i) Apoptosis in TSCCA-CDDP and CAL-27-CDDP cells transfected by si-HOMA11-AS or its negative control was determined using TUNEL assay (p=0.029; p=0.028). (j) Caspase 3 activities in TSCCA-CDDP and CAL-27-CDDP cells transfected by si-HOMA11-AS or its negative control were examined using a specific assay kit (p=0.001; p=0.002). #P < 0.05.