Gossypol Promotes Wnt/-Catenin Signaling through WIF1 in Ovariectomy-Induced Osteoporosis

Figure 3

Gossypol inhibited the expression of WIF1 through its promoter. (a) Gossypol treatment decreased the luciferase activity of WIF1 in 293T cells. (b) The promoter structure of WIF1 from Atlas database. (c) ChIP-PCR assays detected the effects of gossypol on methylation of WIF1. (d) ChIP-qPCR assays detected the effects of gossypol on methylation of WIF1. P<0.05, vs. control.

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