Stimulatory Effects of Boron Containing Bioactive Glass on Osteogenesis and Angiogenesis of Polycaprolactone: In Vitro Study

Table 1

Primer sequences used for rat BMSCs.

Gene	Primers (F=forward; R=reverse)	Accession	Product
		numbers	size (bp)
RUNX2	F: 5′ATCCAGCCACCTTCACTTACACC3′	NM_053470.2	199
	R: 5′GGGACCATTGGGAACTGATAGG3′
BMP-2	F: 5′GAAGCCAGGTGTCTCCAAGAG3′	NM_017178.1	122
	R: 5′GTGGATGTCCTTTACCGTCGT3′
COL1	F: 5′CTGCCCAGAAGAATATGTATCACC3′	NM_053304.1	198
	R: 5′ GAAGCAAAGTTTCCTCCAAGACC3′
BSP	F: 5′AGAAAGAGCAGCACGGTTGAGT3′	NM_012587.2	175
	R: 5′ GACCCTCGTAGCCTTCATAGCC3′
OPN	F: 5′CCAAGCGTGGAAACACACAGCC3′	NM_012881.2	165
	R: 5′GGCTTTGGAACTCGCCTGACTG3′
OCN	F: 5′CAGTAAGGTGGTGAATAGACTCCG3′	NM_013414.1	172
	R: 5′GGTGCCATAGATGCGCTTG3′
VEGF	F: 5′GGCTCTGAAACCATGAACTTTCT3′	NM_001110334.1	165
	R: 5′GCAGTAGCTGCGCTGGTAGAC3′
ANG-1	F: 5′GGACAGCAGGCAAACAGAGCAGC3′	NM_053546.1	130
	R: 5′CCACAGGCATCAAACCACCAACC3′
GAPDH	F: 5′CCTGCACCACCAACTGCTTA3′	NM_017008.4	120
	R: 5′GGCCATCCACAGTCTTCTGAG3′