RETRACTED: HIF-1-miR-219-SMC4 Regulatory Pathway Promoting Proliferation and Migration of HCC under Hypoxic Condition

Figure 2

Suppression of SMC4 induces G1 phase arrest and inhibits process of EMT. (a) Flow cytometry was used to analyze the percentage of different cell cycle phases in HCC cells with SMC4 siRNA and negative control under hypoxic condition. The result of statistical analysis was shown by Histogram. And the expression changes of CDK2, CDK4, and CyclinB1 were detected by western blot, and β-actin was used for internal control. n = 3, . (b) IF and western blot were used to detect expression changes of E-cadherin and Vimentin under hypoxia in HCC cells with SMC4 siRNA and negative control, and β-actin was used for internal control.

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