A Novel OsteomiRs Expression Signature for Osteoblast Differentiation of Human Amniotic Membrane-Derived Mesenchymal Stem Cells

Figure 2

Expression profiling of miRNAs modulated during osteoblastic differentiation of hAM-hMSCs. (a) Unsupervised hierarchical clustering analysis displaying the differential expression of miRNAs at 14 days and (b) 7 days of osteoblastic differentiation relative to time 0 (undifferentiated hAM-MSCs). The heatmap (Spearman correlation; Euclidean distance) represents a cluster analysis of the logarithm of transformed ΔΔCt values of the differentially expressed microRNAs. Color key: upregulated miRNAs (red); downregulated miRNAs (green). (c and d) Volcano plot representations of miRNAs modulated at 7 and 14 days. The y-axis represents the mean expression value of log10 (P-value) and the x-axis displays the log2-fold change value. Upregulated and downregulated miRNAs are shown in red and green, respectively. Black dots indicate genes with no significant change in expression.

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