Paeoniflorin Inhibits Hepatocyte Growth Factor- (HGF-) Induced Migration and Invasion and Actin Rearrangement via Suppression of c-Met-Mediated RhoA/ROCK Signaling in Glioblastoma

Figure 4

Paeoniflorin inhibited HGF-induced RhoA/ROCK signaling activity. T98G and U251 cells were pretreated with or without 10μM paeoniflorin and after that the cells were stimulated with 20ng/ml HGF for 6 hours; then activity of GTP-RhoA (a-d) and the protein expression of ROCK1, Limk1, and p-Limk1 (e-h) were detected. Control: no treatment; PF: 10μM paeoniflorin treatment for 24h. HGF: 20ng/ml HGF treatment for 24h; PF+HGF: 20ng/ml HGF combined with 10μM paeoniflorin incubation for 24h. All tests were performed in triplicate and presented as mean ± standard error. P < 0.05, compared with control group. P < 0.05, compared with HGF group.

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