The Coumarin Derivative 5′-Hydroxy Auraptene Suppresses Osteoclast Differentiation via Inhibiting MAPK and c-Fos/NFATc1 Pathways

Figure 3

5′-HA inhibits RANKL-induced NF-kB and c-Fos/NFATc1 pathway. (a) 5′-HA inhibits RANKL-induced NF-κB signaling activity. BM cells were transfected with Cignal NF-κB reporter negative control or positive control and without (Ctrl) or with RANKL and M-CSF in the absence (0) or the presence of different concentrations of 5′-HA for 24 hours. Reporter activity was represented after normalization to the internal Renilla reporter. (b) Western blot analysis of C-Fos and NFATc1 protein expression. Cells were pretreated with 5′-HA (50 μM) for 1 h and then stimulated with RANKL for 20 min. (c) qPCR and Western blot analysis of TRAF6 expression in RANKL-induced BM cells in the absence and the presence of different concentrations of 5′-HA. Values are mean ± SD of three independent experiments (, , as compared to RANKL-induced cells without 5′-HA).

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