The Coumarin Derivative 5′-Hydroxy Auraptene Suppresses Osteoclast Differentiation via Inhibiting MAPK and c-Fos/NFATc1 Pathways

Figure 5

5′-HA inhibits RANKL-induced MAPKs signaling pathway in BM cells. (a) Western blot analysis of ERK1/2, JNK, and p-38 phosphorylation in RANKL-induced BM cells in the absence and the presence of 5′-HA. Cells were pretreated with 5′-HA (50 μM) for 1 h and then stimulated with RANKL for 20 min. (b) Effect of blocking MAPKs on 5′-HA-inhibited osteoclastogenesis. BM cells were pretreated with specific inhibitors for ERK1/2 (U0126, 10 μM), JNK (SP600125, 10 μM), and p38 (SB203580, 10 μM) and then induced with RANKL and M-CSF in the absence or the presence of 5′-HA. Quantitative TRAP activity was measured after 7 days of treatment and values were presented as percentage of control noninduced. Values are mean ± SD of three independent experiments (, , as compared to RANKL-induced cells without 5′-HA).

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