IgE, IgG1, and IgG4 Reactivity to Dermatophagoides pteronyssinus Glycosylated Extract in Allergic Patients

Figure 5

IgE (a), IgG1 (b), and IgG4 (c) inhibition curves using D. pteronyssinus (Dpt) crude extract on the solid phase, determined by ELISA. Pooled sera from DP+ patients were preabsorbed with different concentrations of Dpt, ConA-unbound, ConA-bound, Df crude extract, and Bt crude extract as inhibitors. Tetanus toxoid (TT) was used as unrelated antigen. Dashed line represents the half-maximal inhibitory concentration (IC₅₀).

(a) IgE

(b) IgG1

(c) IgG4