Identification of Functional Interactome of Gastric Cancer Cells with Helicobacter pylori Outer Membrane Protein HpaA by HPLC-MS/MS

Figure 2

SDS-PAGE analysis of HpaA protein expression in BL21 (DE3). Lane M: molecular weight marker (15, 25, 35, 45, 60, 80, 100, 140, 180) ×10³; lane 1: induced control strain BL21 (DE3) with 0.5 mM IPTG; lane 2: noninduced HpaA; lane 3: induced HpaA with 0.5 mM IPTG; lanes 4, 5, and 6: affinity-purified HpaA (lane 6 corresponds to peak fraction eluted).