Research Article
Identification of Functional Interactome of Gastric Cancer Cells with Helicobacter pylori Outer Membrane Protein HpaA by HPLC-MS/MS
Table 2
List of HpaA-binding proteins identified by HPLC-MS/MS.
(a) List of HpaA-binding proteins from AGS cells identified by HPLC-MS/MS |
| | Accession | Description | Score | Coverage | Proteins | Unique peptides | Peptides | PSMs | AAs | MW (kDa) | Calc. pI |
| | P07355 | Annexin A2 OS=Homo sapiens GN=ANXA2 PE=1 SV=2 - [ANXA2_HUMAN] | 11.28 | 17.99 | 2 | 5 | 5 | 5 | 339 | 38.6 | 7.75 | | Q9Y224 | UPF0568 protein C14orf166 OS=Homo sapiens GN=C14orf166 PE=1 SV=1 - [CN166_HUMAN] | 4.27 | 11.89 | 1 | 2 | 2 | 2 | 244 | 28.1 | 6.65 | | P52815 | 39S ribosomal protein L12, mitochondrial OS=Homo sapiens GN=MRPL12 PE=1 SV=2 - [RM12_HUMAN] | 3.61 | 12.12 | 1 | 1 | 1 | 1 | 198 | 21.3 | 8.87 | | Q7Z5L9 | Interferon regulatory factor 2-binding protein 2 OS=Homo sapiens GN=IRF2BP2 PE=1 SV=2 - [I2BP2_HUMAN] | 2.51 | 1.53 | 1 | 1 | 1 | 1 | 587 | 61.0 | 8.69 | | Q06830 | Peroxiredoxin-1 OS=Homo sapiens GN=PRDX1 PE=1 SV=1 - [PRDX1_HUMAN] | 2.44 | 9.05 | 1 | 2 | 2 | 2 | 199 | 22.1 | 8.13 | | Q9H0L4 | Cleavage stimulation factor subunit 2 tau variant OS=Homo sapiens GN=CSTF2T PE=1 SV=1 - [CSTFT_HUMAN] | 1.98 | 2.11 | 1 | 1 | 1 | 1 | 616 | 64.4 | 7.25 | | Q13573 | SNW domain-containing protein 1 OS=Homo sapiens GN=SNW1 PE=1 SV=1 - [SNW1_HUMAN] | 1.89 | 2.80 | 1 | 1 | 1 | 1 | 536 | 61.5 | 9.52 | | Q15691 | Microtubule-associated protein RP/EB family member 1 OS=Homo sapiens GN=MAPRE1 PE=1 SV=3 - [MARE1_HUMAN] | 1.72 | 5.60 | 1 | 2 | 2 | 2 | 268 | 30.0 | 5.14 | | P35637 | RNA-binding protein FUS OS=Homo sapiens GN=FUS PE=1 SV=1 - [FUS_HUMAN] | 0.00 | 1.71 | 2 | 1 | 1 | 1 | 526 | 53.4 | 9.36 |
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(b) List of HpaA-binding proteins from SGC-7901 cells identified by HPLC-MS/MS |
| | Accession | Description | Score | Coverage | Proteins | Unique peptides | Peptides | PSMs | AAs | MW (kDa) | Calc. pI |
| | P07355 | Annexin A2 OS=Homo sapiens GN=ANXA2 PE=1 SV=2 - [ANXA2_HUMAN] | 13.65 | 17.70 | 2 | 6 | 6 | 6 | 339 | 38.6 | 7.75 | | P27816 | Microtubule-associated protein 4 OS=Homo sapiens GN=MAP4 PE=1 SV=3 - [MAP4_HUMAN] | 4.73 | 2.26 | 1 | 2 | 2 | 2 | 1152 | 120.9 | 5.43 | | P68104 | Elongation factor 1-alpha 1 OS=Homo sapiens GN=EEF1A1 PE=1 SV=1 - [EF1A1_HUMAN] | 4.24 | 4.98 | 3 | 2 | 2 | 2 | 462 | 50.1 | 9.01 | | Q15637 | Splicing factor 1 OS=Homo sapiens GN=SF1 PE=1 SV=4 - [SF01_HUMAN] | 3.76 | 3.76 | 1 | 2 | 2 | 2 | 639 | 68.3 | 8.98 | | P31949 | Protein S100-A11 OS=Homo sapiens GN=S100A11 PE=1 SV=2 - [S10AB_HUMAN] | 2.43 | 8.57 | 1 | 1 | 1 | 1 | 105 | 11.7 | 7.12 | | P04792 | Heat shock protein beta-1 OS=Homo sapiens GN=HSPB1 PE=1 SV=2 - [HSPB1_HUMAN] | 2.18 | 8.29 | 1 | 1 | 1 | 1 | 205 | 22.8 | 6.40 | | P13639 | Elongation factor 2 OS=Homo sapiens GN=EEF2 PE=1 SV=4 - [EF2_HUMAN] | 2.14 | 2.45 | 1 | 2 | 2 | 2 | 858 | 95.3 | 6.83 | | P04406 | Glyceraldehyde-3-phosphate dehydrogenase OS=Homo sapiens GN=GAPDH PE=1 SV=3 - [G3P_HUMAN] | 1.97 | 2.39 | 1 | 1 | 1 | 1 | 335 | 36.0 | 8.46 | | P29401 | Transketolase OS=Homo sapiens GN=TKT PE=1 SV=3 - [TKT_HUMAN] | 1.86 | 3.05 | 1 | 1 | 1 | 1 | 623 | 67.8 | 7.66 | | Q96HC4 | PDZ and LIM domain protein 5 OS=Homo sapiens GN=PDLIM5 PE=1 SV=5 - [PDLI5_HUMAN] | 1.79 | 2.35 | 1 | 1 | 1 | 2 | 596 | 63.9 | 8.21 | | Q15691 | Microtubule-associated protein RP/EB family member 1 OS=Homo sapiens GN=MAPRE1 PE=1 SV=3 - [MARE1_HUMAN] | 1.70 | 13.81 | 1 | 3 | 3 | 3 | 268 | 30.0 | 5.14 | | P13073 | Cytochrome c oxidase subunit 4 isoform 1, mitochondrial OS=Homo sapiens GN=COX4I1 PE=1 SV=1 - [COX41_HUMAN] | 1.70 | 5.92 | 1 | 1 | 1 | 1 | 169 | 19.6 | 9.51 | | P04264 | Keratin, type II cytoskeletal 1 OS=Homo sapiens GN=KRT1 PE=1 SV=6 - [K2C1_HUMAN] | 1.64 | 3.73 | 3 | 2 | 2 | 2 | 644 | 66.0 | 8.12 |
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PSM: peptide spectrum match; AA: length of protein; MW: molecular weight; calc. pI: calculated isoelectric point. |
