Research Article
Silencing of PRDX2 Inhibits the Proliferation and Invasion of Non-Small Cell Lung Cancer Cells
Figure 1
Knockdown of PRDX2 inhibits the proliferation of NSCLC cells in vitro. (a) Expression of PRDX2 mRNA in BEAS-2B, A549, and H1299 cells was determined using qRT-PCR assay. A549 and H1299 cells were transfected with siRNA-PRDX2 (si-PRDX2), and siRNA negative control was used as negative control (si-NC). (b) Following transfection of 24 h, the expression of PRDX2 mRNA was determined using qRT-PCR assay. (c) The relative expression of PRDX2 protein in NSCLC cells transfected with siRNAs for 48 h was examined using western blot analysis. (d, e) CCK8 assay was performed the viability of A549 (d) and H1299 (e) cells. (f) Colony formation assay was used to assess cell proliferation. (g) Quantitative analysis of the results of colony formation assay. (h) The expression of PRDX2 protein in A549 cells transfected with PRDX2 inhibitor was examined using western blot analysis. (i) CCK8 assay was performed to measure A549 cell viability after indicated treatment. (j) Colony formation assay was used to assess A549 cell proliferation. , .
(a) |
(b) |
(c) |
(d) |
(e) |
(f) |
(g) |
(h) |
(i) |
(j) |