Inhibition of DNA Repair Protein Ku70 in High-Glucose Environment Aggravates the Neurotoxicity Induced by Bupivacaine in SH-SY5Y Cells

Figure 6

High glucose enhanced bupivacaine-induced DNA damage and cell apoptosis, which was attenuated by the Ku70 overexpression. (a) DNA damage as detected by the cosmet assay and the Olive tail moment. (b) Cells in early apoptosis are annexin V-FITC-positive (right upper quadrant) and PI-negative (right lower quadrant). Summarized data show the early apoptotic rates as detected by flow cytometry. (c) Cell apoptosis as detected by the TUNEL assay. Con: untreated SH-SY5Y cells; HG: SH-SY5Y cells exposed to 50 mM glucose for 7 days; Bup: SH-SY5Y cells treated with 1.0 mM bupivacaine for 24 h; HG + Bup: SH-SY5Y cells incubated with 50 mM glucose for 7 days before treatment with 1.0 mM bupivacaine for 24 h; LV5 + HG + Bup: SH-SY5Y cells transfected vector (LV5) incubated with 50 mM glucose for 7 days before treatment with 1.0 mM bupivacaine for 24 h; LV5-Ku70 + HG + Bup: SH-SY5Y cells transfected overexpression lentivirus (LV5-Ku70) incubated with 50 mM glucose for 7 days before treatment with 1.0 mM bupivacaine for 24 h. Values are the mean ± SEM of n = 3. compared with Con. compared with HG and Bup. compared with HG + Bup and LV5 + HG + Bup.

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