Cytokines Induce Monkey Neural Stem Cell Differentiation through Notch Signaling

<div>Monkey NSCs expressed functional BMP signaling machinery after BMP4 treatment. (a) BMP signaling pathway relative genes were detected in monkey NSCs. BMPR2, Smad4, Id1, and Id2 were upregulated in BMP4-treated cells compared with control. Data represent the mean ± SD. (b) BMP target genes MSX2, GSK3<i>β</i>, TKT, and Hif1<i>α</i> were analyzed after BMP4 treatment in monkey NSCs. Expression levels of these genes were significantly higher after treatment with BMP4 except Hif1<i>α</i>. Hif1<i>α</i> showed no response to BMP. Data represent the mean values ± SD. (c) Genes of the Notch pathway were tested such as Notch1, Notch2, Notch3, Notch4, Hes1, and Hes5. BMP4 had strongly promoted the expression level of Notch3, Notch 4, Hes1, and Hes5 in monkey NSCs. 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BioMed Research International

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Figure 2

Figure 2: Cytokines Induce Monkey Neural Stem Cell Differentiation through Notch Signaling 