Cytokines Induce Monkey Neural Stem Cell Differentiation through Notch Signaling

Figure 5

NSCs could differentiate into astrocyte-like and neuron-like after BMP4/LIF or RA/forskolin treatment. (a) BMP4/LIF or RA/forskolin induced monkey NSC differentiation. BMP4/LIF promoted monkey NSCs to astrocyte-like cells and RA/forskolin encouraged monkey NSCs to neuron-like cells. (b) Higher magnification of (a). (c) Ratio of positive cells in monkey NSCs induced by BMP4/LIF or RA/Forskolin. Cell number was assessed in 6 random fields of each well (3 wells/group). Data represent the mean ± SD. was considered statistically significant. (d) Cell morphology showed that BMP4/LIF or RA/forskolin induced the differentiation of HCN cells. The right column is the higher magnification of the area with a white frame in the middle. (e) BMP4/LIF also promoted rat hippocampal neural stem cells to astrocyte-like cells and RA/forskolin encouraged rat hippocampal neural stem cells to neuron-like cells. Neural stem cells were stained with Sox2, GFAP, and Tuj1. The right column is the higher magnification of the area with a white frame in the middle. All scale bars = 50 μm.

(a)

(b)

(c)

(d)

(e)