Cytokines Induce Monkey Neural Stem Cell Differentiation through Notch Signaling

Figure 6

BMP4/LIF and RA/forskolin may promote monkey NSC differentiation by inducing the Notch signaling pathway. (a) BMP4/LIF induced the expression of Notch signaling pathway genes and GFAP. BMP4/LIF increases the expression of genes downstream of the Notch pathway and Jak/Stat pathway such as Notch1-4, Dll1, Jag2, Hes1, Hes5, Jak1, Jak2, and Stat3. (b) Neural differentiation-related genes were analyzed, such as Tuj1, DCX, and MAP2, Notch pathway genes Jag1 and Hes1, proneural genes TLX, ASCL1, and Mef2c, and differentiation repressor genes HDAC4 and REST. High expression levels of Tuj1, MAP2, and DCX appeared after RA and forskolin treatment. RA/forskolin reduced the expression levels of Jag1 and Hes1. Expression levels of TLX, ASCL1, and MEF2C were significantly increased, whereas HDAC4 and REST depressed during neuronal differentiation in monkey NSCs. Data represent the mean ± SD. were considered statistically significant.

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