Screening of Concentration and Antimicrobial Effectiveness of Antimicrobial Preservative in Betastatin Besylate Nasal Spray

Table 3

Results of antimicrobial effectiveness.

Nasal spray no.	Bacteriostatic concentration	Test strains	Count of bacteria in the specimen (CFU/mL)					Reduced log value
			Initial value	2 d	7 d	14 d	28 d	Initial value	2 d	7 d	14 d	28 d	28 d compared with 7 d	28 d compared with 14 d
71401	0.02% benzalkonium chloride	S. aureus		<1	<1	<1	<1	6.3	6.3	6.3	6.3	6.3	NI	NI
		P. aeruginosa		<1	<1	<1	<1	6.8	6.8	6.8	6.8	6.8	NI	NI
		E. coli		<1	<1	<1	<1	6.6	6.6	6.6	6.6	6.6	NI	NI
		C. albicans		—	—	<1	<1	5.6	—	—	5.6	5.6	—	NI
		A. niger		—	—	<1	<1	5.0	—	—	5.0	5.0	—	NI
71402	0.0125% benzalkonium chloride	S. aureus		<1	<1	<1	<1	6.3	6.3	6.3	6.3	6.3	NI	NI
		P. aeruginosa		<1	<1	<1	<1	6.8	6.8	6.8	6.8	6.8	NI	NI
		E. coli		<1	<1	<1	<1	6.6	6.6	6.6	6.6	6.6	NI	NI
		C. albicans		—	—	<1	<1	5.6	—	—	5.0	5.0	—	NI
		A. niger		—	—	<1	<1	5.0	—	—	4.7	4.7	—	NI
71403	0.005% benzalkonium chloride	S. aureus		<1	<1	<1	<1	6.3	6.3	6.3	6.3	6.3	NI	NI
		P. aeruginosa		<1	<1	<1	<1	6.8	6.8	6.8	6.5	6.5	-2.1^a	-1.8^b
		E. coli		<1	<1	<1	<1	6.6	6.6	6.6	6.6	6.6	NI	NI
		C. albicans		—	—	<1	<1	5.6	—	—	1.7	1.7	—	NI
		A. niger		—	—	<1	<1	5.0	—	—	5.0	5.0	—	NI

NI: no increase means that the increase in test strain did not exceed 0.5 log from the previous measurement time; ^a indicates that the increase in test strain was 2.1 log, when compared with that on days 7 and 28; ^b denotes that result of day 28 is comparable with that of day 14, and the increase in the number of test strain was 1.8 log.