Accumulation of AGO2 Facilitates Tumorigenesis of Human Hepatocellular Carcinoma

Figure 2

AGO2 promoted cell proliferation and migration in HCC in vitro. (a) AGO2 protein abundances in different cell lines were detected by western blot analysis; GAPDH was used as a loading control. (b) The ratio of AGO2 to GAPDH in each cell line was measured by densitometric analysis of western blot bands, respectively. (c) Designation of AGO2 protein depletion in SMMC-7721 cells using CRISPR/Cas9 gene editing strategy. (d) AGO2 expression in SMMC-7721 cells was determined by western blotting. (e) Colony formation detected by Giemsa staining for normal and AGO2^-/- SMMC-7721 cells. (f) AGO2 knockout induced inhibition in colony formation as compared to control. (g) Diagram of the cell cycle analysis for normal and AGO2^-/- SMMC-7721 cells based on flow cytometry. (h) The proportions of cells in G1, S, and G2 phases were calculated. (i) Representative images for cell migration assay. (j) Numbers of stained cells in migration assay were counted. Data are normalized and expressed as the fold change relative to control values. Values represented as the ( each group); , , and .

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