Accumulation of AGO2 Facilitates Tumorigenesis of Human Hepatocellular Carcinoma

Figure 6

AGO2 overexpression promoted proliferation and migration of SMMC-7721 and Huh-7 in vitro. (a–f) Huh-7 cells were transiently transfected with pCMV3-AGO2 (AGO2^OE) and an empty vector pCMV3 (control), respectively. (g–l) SMMC-7721 cells were used for AGO2 overexpression and subsequently analysis. Western blot was used to examine the effect of pCMV3-AGO2 transfection into Huh-7 (a) and SMMC-7721 (g) cells. (b and h) CCK8 assay was performed to detect cell proliferation of AGO2^OE Huh-7 (b) and SMMC-7721 (h) cells. (c and i) Cell migration rates of AGO2^OE Huh-7 (c) and SMMC-7721 (i) were determined by transwell assay. Western blot was performed to detect the effect of AGO2 overexpression on Survivin, Snail, and Vimentin expression in Huh-7 (d–f) and SMMC-7721 (j–l) cells. The ratio of Survivin, Snail, and Vimentin to GAPDH in Huh-7 and SMMC-7721 was measured by densitometric analysis of western blot bands, respectively. Values represented as the ( each group); , , and .

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