Neuroepithelial Cell Transforming Gene 1 Acts as an Oncogene and Is Mediated by miR-22 in Human Non-Small-Cell Lung Cancer

Figure 5

Overexpression of NET1 without 3′UTR rescued the roles of miR-22 in cell proliferation, migration, and apoptosis in NSCLC cells. (a, b) The expression level of miR-22 and its target gene NET1 was evaluated by using qRT-PCR and western blot, respectively (n = 3). (c, d) Overexpression of NET1 counteracted the inhibition of the cell vitality and growth capacity using CCK-8 and EdU assays (n = 4). (e) The suppression of migration of A549 and H1299 cells could be reversed by NET1 vector using wound healing assay (n = 3). (f) Analogously, NET1 vector neutralized the proapoptotic effect of miR-22 via using western blotting analyses (n = 3). .

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