GFAT1/HBP/O-GlcNAcylation Axis Regulates β-Catenin Activity to Promote Pancreatic Cancer Aggressiveness

Figure 3

GFAT1 knockdown or inhibition suppresses PDAC aggressiveness. (a) Western blotting analysis of the GFAT1 protein level in PDAC cell lines; quantification data was shown, and the β-actin was used as an internal control. (b) Western blotting analysis of the knockdown efficiency of GFAT1 in BxPC3 and SW1990 cells; quantification data was shown, and the β-actin was used as an internal control. (c) The effect of GFAT1 knockdown on BxPC3 and SW1990 cell proliferation was determined by CCK-8 assay. (d) The effect of GFAT1 knockdown on BxPC3 and SW1990 cell apoptosis was determined by caspase-3/7 activity assay. (e) The effect of GFAT1 knockdown on BxPC3 and SW1990 cell invasion was determined by transwell assay. (f) The effect of GFAT1 inhibition on BxPC3 and SW1990 cell proliferation was determined by CCK-8 assay. (g) The effects of GFAT1 inhibition on BxPC3 and SW1990 cell apoptosis and invasion were determined by caspase-3/7 activity assay and transwell assay, respectively. ; .

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