GFAT1/HBP/O-GlcNAcylation Axis Regulates <i>β</i>-Catenin Activity to Promote Pancreatic Cancer Aggressiveness

<div>GFAT1 is induced by the PI3K/AKT/mTOR pathway and promotes <i>β</i>-catenin activity by O-GlcNAcylation. (a) Correlation analysis of HBP gene signatures and PI3K/AKT/mTOR and Wnt/<i>β</i>-catenin signaling gene signatures. (b) The effects of LY294002 (20 <i>μ</i>M), rapamycin (50 nM), and ICG-001 (5 <i>μ</i>M) on the mRNA expression of GFAT1 in BxPC3 and SW1990 cells were analyzed by real-time qPCR. (c) Western blotting analysis of the effect of GFAT1 knockdown on the PI3K/AKT/mTOR signaling in BxPC3 and SW1990 cells; quantification data was shown, and the <i>β</i>-actin was used as an internal control. (d) Western blotting analysis of the effect of GFAT1 knockdown on the <i>β</i>-catenin O-GlcNAcylation; quantification data was shown, and the <i>β</i>-actin was used as an internal control. <span class="nowrap"><svg height="10.7395pt" id="M39" style="vertical-align:-0.6370001pt" version="1.1" viewbox="-0.0498162 -10.1025 50.9184 10.7395" width="50.9184pt" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink"><g transform="matrix(.0091,0,0,-0.0091,0,-5.741)"><path d="M486 158C486 177 478 202 466 220C413 228 386 236 336 262C386 288 413 297 466 304C478 323 486 347 485 366C470 376 444 381 422 380C389 338 368 319 321 288C323 345 329 372 349 422C339 442 322 461 305 470C289 461 271 442 262 422C281 372 287 345 290 288C243 319 222 338 189 380C167 381 142 376 125 366C125 347 133 322 145 304C198 296 225 288 275 262C225 236 198 227 145 220C133 201 125 177 126 158C141 148 167 143 189 144C222 186 243 205 290 236C288 179 282 152 262 102C272 82 289 63 306 54C322 63 340 82 350 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BioMed Research International

fig4

Figure 4

Figure 4: GFAT1/HBP/O-GlcNAcylation Axis Regulates <i>β</i>-Catenin Activity to Promote Pancreatic Cancer Aggressiveness 

BioMed Research International

GFAT1/HBP/O-GlcNAcylation Axis Regulates β-Catenin Activity to Promote Pancreatic Cancer Aggressiveness

Figure 4