Ginkgetin Alleviates Inflammation, Oxidative Stress, and Apoptosis Induced by Hypoxia/Reoxygenation in H9C2 Cells via Caspase-3 Dependent Pathway

Figure 3

Ginkgetin attenuates inflammation induced by H/R through caspase-3 dependent pathway. (a) Cytochrome C, caspase-3, and pro-caspase-3 were estimated by western blot analysis. (b) Cytochrome C was evaluated using an assay kit. (c) H9C2 cells were treated with 0.6 μM 2-HBA, and then, cytochrome C, caspase-3, and pro-caspase-3 were estimated via western blot analysis. (d) Assay kit was employed to determine the level of cytochrome C. (e–h) TNF-α, IL-6, IL-1β, and HMGB1 were determined using assay kits. versus control group; , versus vehicle group; , , versus ginkgetin group.

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