Research Article
Ginkgetin Alleviates Inflammation, Oxidative Stress, and Apoptosis Induced by Hypoxia/Reoxygenation in H9C2 Cells via Caspase-3 Dependent Pathway
Figure 4
Ginkgetin ameliorates H/R-triggered oxidative stress and apoptosis via caspase-3 dependent pathway. (a) Western blot analysis was conducted to assess the protein expression of NF-κB. (b–d) MDA, SOD, and LDH were detected using assay kits. (e) Levels of Nox2 and Nox4 were detected via western blot analysis. (f, g) Flow cytometry analysis was employed to screen apoptotic rate. (h) Bcl-2, Bax, caspase-9, and pro-caspase-9 were estimated by western blot analysis. versus control group; , versus vehicle group; , , versus ginkgetin group.
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