CUMS Promotes the Development of Premature Ovarian Insufficiency Mediated by Nerve Growth Factor and Its Receptor in Rats

Table 2

Real-time PCR amplification system and reaction conditions.

Reagent component	20 μL system	Reaction conditions
SDW	8.0 μL	95°C, 1 min; (95°C, 15 sec, 60°C, 40 sec) 40 cycles; 55 to 95°C melting curve
Power SYBR green master mix	10.0 μL
Forward primer(10 μM)	0.5 μL
Reverse primer(10 μM)	0.5 μL
cDNA	1.0 μL