Inhibition of MEIS3 Generates Cetuximab Resistance through c-Met and Akt

Figure 5

(a) Protein levels of LOVO-CON, MEIS3 shRNA, MEIS3 shRNA+c-Met overexpression, MEIS3 shRNA+c-Met shRNA, and MEIS3 overexpression+c-Met shRNA were extracted, and the expression of p-EGFR, EGFR, p-Akt, Akt, p-c-Met, and c-Met at the protein level was detected by western blot. (b) The LOVO and LOVO MEIS3 shRNA were treated with CHX for different times, and the expression of Akt was detected by western blot. (c) The SNU-61 cell line was treated with CHX for different times, and the expression of Akt was detected by western blot. (d) The LOVO cells were first treated with CHX followed by DMSO, BAF, and MG132 for different times, and the expression of Akt was determined by western blot. (e) SNU-61 (A) and HCT-116 (B) were transfected with CON and MEIS3 shRNA and then treated with DMSO, MG132, and BAF, and the degradation of Akt was determined by western blot. (f) LOVO (A) and SNU-61 (B) were transfected with the CON and MEIS3 vectors, and the ubiquitin-bonded Akt was detected using IP. (g) The LOVO cell line was transfected with random shRNA, CON vector, and c-Met shRNA for 48 h, and then, western blot was used to detect the change of c-Met in the protein level.

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