Research Article
Cloning and Functional Determination of Ammonium Transporter PpeAMT3;4 in Peach
Table 1
Primer sequences used in this work.
| | Purpose | Primer (5-3) | Amplicon (bp) |
| | Amplication of PpeAMT3;4 CDS | F: ATGATGCGAGGTTGGTGC
R: TTAGACCACCTGAGTGGCA | 1065 | | Specific expression primers of PpeAMT3;4 | F: TGTCGGAGCTTTCAGCTTGT
R: TGAAACCTGCCCATCCCATC | 224 | | Specific expression primers of Ubiquitin | F: AGGCTAAGATCCAAGACAAAGA
R: CCACGAAGACGAAGCACTAAG | 145 | | Construction of pYES2-AMT3;4 plasmid | F: GAGCGGTACCATGATGCGAGGTTGGTGC
R: GCGAGCGGCCGCTTAGACCACCTGAGTGGC | 1065 |
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