RRS1 Promotes Retinoblastoma Cell Proliferation and Invasion via Activating the AKT/mTOR Signaling Pathway

Figure 3

RRS1 regulates the cell cycle, apoptosis, and invasion of RB cells. (a, b) RRS1 knockdown and overexpressing Y79 cells and the respective controls were subjected to flow cytometry analysis of cell cycle distribution. (a) FCS images of cell cycle. (b) Quantification of cell cycle distribution. (c, d) RRS1 knockdown and overexpressing WERI-Rb-1 cells and the respective controls were subjected to flow cytometry analysis of cell cycle distribution. (c) FCS images of cell cycle. (d) Quantification of cell cycle distribution. (e, f) RRS1 knockdown and overexpressing Y79 cells and the respective controls were subjected to apoptosis analysis. (e) FCS images of cell apoptosis. (f) Quantification of cell apoptosis. , . (g, h) RRS1 knockdown and overexpressing WERI-Rb-1 cells and the respective controls were subjected to apoptosis analysis. (g) FCS images of cell apoptosis. (h) Quantification of cell apoptosis. , . (i) Transwell analysis of invaded Y79 cells transfected with shCtrl, shRRS1-1, and shRRS1-2 or with control and RRS1 overexpression lentiviruses. , . (j) Transwell analysis of invaded WERI-Rb-1 cells transfected with shCtrl, shRRS1-1, and shRRS1-2 or with control and RRS1 overexpression lentiviruses. .