RRS1 Promotes Retinoblastoma Cell Proliferation and Invasion via Activating the AKT/mTOR Signaling Pathway

Figure 4

RRS1 activates the AKT/mTOR signaling pathway. (a) Western blot analysis of AKT and mTOR phosphorylation in RRS1 knockdown and the control and in RRS1 overexpressing and the control Y79 cells. (b) Control and RRS1 overexpressing Y79 cells treated with or without MK-2206 were subjected to western blot analysis with the indicated antibodies. (c) Control and RRS1 overexpressing Y79 cells were treated with or without MK-2206, and the cell viability was analyzed by CCK8 assay. , . (d) Control and RRS1 overexpressing Y79 cells were treated with or without MK-2206 and then subjected to colony formation analysis. (e) Control and RRS1 overexpressing Y79 cells were treated with or without MK-2206 and then subjected to Transwell analysis of invaded cells. . (f) Control and RRS1 overexpressing WERI-Rb-1 cells were treated with or without MK-2206 and then subjected to Transwell analysis of invaded cells. . (g) Control and RRS1 overexpressing Y79 cells and RRS1 overexpressing Y79 cells treated with rapamycin were subjected to western blot analysis with the indicated antibodies. (h, i) Control and RRS1 overexpressing Y79 cells and RRS1 overexpressing Y79 cells treated with rapamycin were subjected to CCK8 analysis of cell proliferation (h) and Transwell analysis of invaded cells (i). , .

(a)

(b)

(c)

(d)

(e)

(f)

(g)

(h)

(i)