Cell-to-Cell Spread of Dengue Viral RNA in Mosquito Cells

Figure 5

Delivery of viral RNA from donor to recipient cells in the transwell system. Intercellular delivery of viral RNA was also investigated in the transwell system containing infected (donor) and uninfected (recipient) cells. DENV (-) RNA was detected only in donor cells, either treated with 40 mM NH₄Cl or not, whether they were transfected with HA-C189 or not. On the other hand, viral RNA was identified from recipient cells only in the absence of 40 mM NH₄Cl treatment, further suggesting that DENV2 can be delivered from donor to recipient cells upon cell-cell contact.