Research Article

Developmental and Tissue Patterns of the Basal Expression of Chicken Avian β-Defensins

Figure 2

Tissue patterns of chicken AvBDs along the GI tract, liver, and spleen. Tissue segments of the esophagus, crop, proventriculus, gizzard, duodenum, ileum, cecum, colon, liver, and spleen were obtained from chickens at 7-day-old. After RNA isolation, RT-qPCR analysis was performed to evaluate the mRNA expression levels of all chicken AvBDs. Fold changes of chicken AvBD expression were calculated relative to the expression level of the colon on day 28 using GAPDH as the reference gene and are expressed as the of the mean of nine chickens.