Developmental and Tissue Patterns of the Basal Expression of Chicken Avian β-Defensins

Figure 4

Developmental expression of chicken β-defensins in the esophagus. Esophagus segments were harvested from broilers at 1, 3, 7, 14, and 28 days of age and were subjected to RT-qPCR analysis after RNA isolation and reverse transcription. The mRNA expression levels of β-defensins at the indicated ages were calculated as fold changes relative to the expression level on day 28 using GAPDH as the housekeeping gene. Each bar represents the of the mean of three chickens. The difference was considered significant using one-way ANOVA followed by Tukey’s test. ; ; .