Detection of Metalloproteases and Cysteine Proteases RNA Transcripts of Leishmania (Leishmania) infantum in Ear Edge Skin of Naturally Infected Dogs

Figure 2

Expression of metalloprotease and cysteine protease genes from Leishmania (L.) infantum in naturally infected dogs. Total RNA from dog’s ear edge skin was extracted and reverse transcription polymerase chain reactions (RT-PCR) were performed using specific primers for the β-actin (a), metalloprotease (b), and cysteine protease (c) genes. RT-PCR products were resolved on a 2% agarose gels stained with Nancy-520. A 100 bp DNA ladder (L) was used as a molecular weight marked and revealed single 87 bp, 202 bp, and 227 bp fragments, respectively, in the tested cDNA samples. Asymptomatic (Dog 2, Dog 4, Dog6, Dog 11, Dog 12, Dog 16, and Dog 19), oligosymptomatic (Dog 3, Dog 5, Dog 7, Dog 8, Dog 9, Dog 10, Dog 13, Dog 14, and Dog 20) and polysymptomatic (Dog 1, Dog 15, Dog 17 and Dog 21).